The Biotechnology section of PBRI is engaged in providing evidence for cellular and molecular biology-based mechanisms for the proposed biological activity of chemicals and matrix preparations from synthetic or natural origins.

Investigations conducted by the biotechnology team are as follows:

• Cytogenetics: Investigation of chemoprotective activity utilizing chromosomal aberration and micronucleus assay
• Animal cell culture: In vitro cytotoxicity and antiproliferative effect on various cell lines (e.g., B16F10, MDA-MB, MCF, 13762NF, etc.)
• Molecular biology: Impact on gene expression (e.g., H-Ras gene expression in skin cancer)
• Enzymology: Quantitative and qualitative estimation of various enzymes involved in vital biochemical reactions (e.g., SOD, LPO, Catalase, GSH, etc.)
• ELISA-based investigation: for critical biochemical mediators (e.g., Interleukins, TNF, C-reactive protein, etc.)
• Nano-technology: Preparation, characterization, and biological/toxicological investigation of nanoparticles